Viral Interference between the Insect-Specific Virus Brejeira and the Saint Louis Encephalitis Virus In Vitro.

The Saint Louis encephalitis virus (SLEV) is an encephalitogenic arbovirus (Flaviviridae family) that has a wide geographical distribution in the western hemisphere, especially in the Americas. The negevirus Brejeira (BREV) was isolated for the first time in Brazil in 2005. This study aimed to verify the existence of a possible interfering effect of BREV on the course of SLEV infection and vice versa. We used clone C6/36 cells. Three combinations of MOIs were used (SLEV 0.1 × BREV 1; SLEV 1 × BREV 0.1; SLEV 1 × BREV 1) in the kinetics of up to 7 days and then the techniques of indirect immunofluorescence (IFA), a plaque assay on Vero cells, and RT-PCR were performed. Our results showed that the cytopathic effect (CPE) caused by BREV was more pronounced than the CPE caused by SLEV. Results of IFA, the plaque assay, and RT-PCR showed the suppression of SLEV replication in the co-infection condition in all the MOI combinations used. The SLEV suppression was dose-dependent. Therefore, the ISV Brejeira can suppress SLEV replication in Aedes albopictus cells, but SLEV does not negatively interfere with BREV replication.

Impact of COVID-19 pandemic on completed treatments and referrals during urgent dental visits.

This ecological study assessed the impact of the COVID-19 pandemic on completed treatments (CTs) and referrals during urgent dental visits to primary health care units in Brazil, and their associations with socioeconomic, geodemographic, and pandemic index factors in Brazilian municipalities. The difference in rates of procedures 12 months before and during the pandemic was calculated. Data were extracted at baseline from health information systems of all municipalities that provided urgent dental care (n = 5,229 out of 5,570). Multiple logistic regression predicted the factors associated with referrals and CTs. The number of dental urgencies increased from 3,987.9 to 4,272.4 per 100,000 inhabitants. The rates of referrals decreased in 44.1% of the municipalities, while 53.9% had lower rates of CTs. Municipalities with a greater number of oral health teams in the primary health care system (OR = 1.52, 95%CI:1.21-1.91) and with specialized services (OR = 1.80, 95%CI:1.50-2.16) were more likely to decrease referrals during the pandemic. Higher HDI and GDP per capita were associated with a larger decrease in referrals and smaller decrease in CTs. The calamity generated by the long pandemic period resulted in a greater demand for urgent visits. Less developed and larger cities seem to have been more likely to not complete treatments during urgent visits in primary dental care units in times of calamity. Primary dental care offices in smaller and less developed municipalities should be better equipped to provide appropriate assistance and to improve the problem-solving capacity of dental services during emergencies.

Impact of COVID-19 pandemic on completed treatments and referrals during urgent dental visits

Abstract This ecological study assessed the impact of the COVID-19 pandemic on completed treatments (CTs) and referrals during urgent dental visits to primary health care units in Brazil, and their associations with socioeconomic, geodemographic, and pandemic index factors in Brazilian municipalities. The difference in rates of procedures 12 months before and during the pandemic was calculated. Data were extracted at baseline from health information systems of all municipalities that provided urgent dental care (n = 5,229 out of 5,570). Multiple logistic regression predicted the factors associated with referrals and CTs. The number of dental urgencies increased from 3,987.9 to 4,272.4 per 100,000 inhabitants. The rates of referrals decreased in 44.1% of the municipalities, while 53.9% had lower rates of CTs. Municipalities with a greater number of oral health teams in the primary health care system (OR = 1.52, 95%CI:1.21-1.91) and with specialized services (OR = 1.80, 95%CI:1.50-2.16) were more likely to decrease referrals during the pandemic. Higher HDI and GDP per capita were associated with a larger decrease in referrals and smaller decrease in CTs. The calamity generated by the long pandemic period resulted in a greater demand for urgent visits. Less developed and larger cities seem to have been more likely to not complete treatments during urgent visits in primary dental care units in times of calamity. Primary dental care offices in smaller and less developed municipalities should be better equipped to provide appropriate assistance and to improve the problem-solving capacity of dental services during emergencies.

Limitação terapêutica para crianças: revisão sistemática sobre final de vida / Therapeutic limitation for children: a systematic review on end-of-life / Limitación terapéutica para niños: una revisión sistemática sobre el final de la vida

Resumo Esta revisão sistemática visa identificar dificuldades enfrentadas por profissionais no manejo clínico de crianças em final de vida. Para tanto, realizou-se busca de artigos científicos nas bases de dados SciELO e LILACS por meio dos descritores "limitação de suporte terapêutico", "terminalidade" e "medidas de conforto", combinados com o descritor "crianças". Do total de 102 trabalhos completos encontrados, nove contemplaram os critérios de inclusão para a amostra do estudo. Os resultados apontam dificuldades em relação à tomada de decisões e à limitação terapêutica de pacientes infantis. Conclui-se que, quando se sentem melhor preparados para trabalhar com questões referentes ao processo de morte e morrer, os profissionais podem prestar um cuidado mais humanizado a pacientes e familiares.

Abstract This systematic review sought to identify difficulties faced by professionals in the clinical management of end-of-life child patients. Bibliographic search was conducted on the SciELO and LILACS databases using the descriptors "therapeutic limitation," "hospice care" and "comfort measures," combined with the descriptor "child." Of the 102 complete studies identified, nine met the established inclusion criteria. The results point to difficulties regarding decision making and therapeutic limitation for infant patients. In conclusion, when professionals feel better prepared to address issues related to the process of death and dying, they can provide more humanized care to patients and families.

Resumen Esta revisión sistemática tiene por objetivo identificar las dificultades que enfrentan los profesionales en el manejo clínico de los niños al final de la vida. Para ello, se realizó una búsqueda de artículos científicos en las bases de datos SciELO y LILACS utilizando las palabras clave "limitación del apoyo terapéutico", "final de la vida" y "medidas de confort", combinadas con "niños". Del total de 102 artículos completos encontrados, nueve cumplieron con los criterios de inclusión para la muestra del estudio. Los resultados apuntan a dificultades con relación a la toma de decisiones y a limitaciones terapéuticas de los pacientes pediátricos. Se concluye que los profesionales cuando se sienten más preparados para lidiar con cuestiones relacionadas con el proceso de muerte y morir pueden brindar una asistencia más humanizada a los pacientes y familias.

Urgent dental care in the Brazilian public health system: learning lessons from the COVID-19 pandemic for future situations.

This ecological study described the effect of the COVID-19 pandemic and socioeconomic development on the use and profile of urgent dental care (UDC). UDC rates per 100,000 inhabitants before (from March to June 2019) and during (from March to June 2020) the COVID-19 pandemic in 4,062 Brazilian municipalities were compared. Data were collected from official sources. COVID-19 mortality and hospitalization rates were indicative of levels of lockdown and Human Development Index (HDI) indicated socioeconomic development. Multiple logistic regression and relative excess risk due to interaction (RERI) were used for statistical analyses. The Student t-test was used to compare changes in the profile of UDC causes and procedures in the two periods. Lower UDC rates were found in 69.1% of municipalities and were associated with higher HDI (OR = 1.20; 95%CI: 1.01; 1.42). Mortality had OR = 0.88 (95%CI: 0.73; 1.06) for municipalities with HDI < 0.70 and OR = 1.45 (95%CI: 1.07; 1.97) for municipalities with HDI > 0.70. RERI between HDI and COVID-19 was 0.13 (p < 0.05). Municipalities with greater primary health care coverage had a smaller reduction in emergency rates. Endodontic treatment and dental pain were the most frequent factors both before and during the pandemic. The percentage of UDCs due to pain and soft tissue damage, as well as temporary sealing and surgical procedures, increased. Socioeconomic variables affected UDC rates during the most restrictive period of the COVID-19 pandemic and should be considered in the planning of health actions in future emergencies.

Hydrolysis of macauba kernel oil: ultrasound application in the substrates pre-emulsion step and effect of the process variables.

The main goal of the present work was to evaluate the application of ultrasound as a previous step to promote the substrates pre-emulsion in the hydrolysis reaction of macauba kernel oil (MKO). The ultrasound effect on the substrates pre-emulsion was evaluated on the free fatty acid (FFA) content, as well as the process variables (reaction time, percentage of catalyst Lipozyme® RM IM, and buffer solution). Reactions carried out with the substrates pre-emulsion presented higher FFA production, up to a 40 wt% increase in 1 hour of reaction, yielding 80 wt% of FFAs in 8 hours. The use of catalyst in the reaction medium, from 5 to 15 wt%, favored the FFAs production in 2 hours of reaction. Addition of 25 to 100 wt% of buffer solution led to 86 wt% of FFAs in 4 hours of reaction. Enzyme recycling resulted in a slight decrease in the FFA content, although the catalyst had maintained 85% of its initial activity after 30 h of use. Therefore, the ultrasound pre-emulsion previous step allowed a more efficient hydrolysis reaction of MKO, leading to an increase of up to 40 wt% on the FFA content, when compared to the hydrolysis without such step.

Prognosis of primary teeth following intrusive luxation according to the degree of intrusion: A retrospective cohort study.

BACKGROUND/AIM: The degree of intrusion is an important aspect to be evaluated when treating children following intrusive luxation. The aim of this retrospective cohort study was to evaluate re-eruption rates and the occurrence of sequelae in intruded primary teeth according to the degree of intrusion. MATERIAL AND METHODS: A total of 238 teeth from 168 patients diagnosed with and treated for intrusion over a 16-year period at a specialized trauma center in South Brazil were included in this study. Patient details, including demographic characteristics, tooth affected, presence of sequelae of trauma during the follow up (pulp necrosis and infection, crown discoloration and abnormal mobility), and any effects on the permanent successors, were collected from their records. Photographs were analyzed to determine the degree of intrusion (Grade I, II, or III) and spontaneous re-eruption during follow up. Re-eruption and occurrence of sequelae according to the degree of intrusion were evaluated using the chi-squared test. The level of significance was set at 5%. RESULTS: Most teeth were maxillary central incisors (87.8%) with Grade I intrusion (32.8%). In the first 30 days, 13.1% had signs of re-eruption. After 3-6 months, the frequency of re-eruption increased to 58.7%. Of the teeth evaluated 6 months or more after intrusion, 68% had re-erupted. Total intrusion (Grade III) was associated with pulp necrosis and infection (p = .002), but the degree of intrusion was not associated with the presence of crown discoloration or abnormal mobility. Of the 78 permanent successors evaluated, 52.6% had developmental disturbances. CONCLUSION: Most intruded primary teeth re-erupted spontaneously, and the degree of intrusion was associated with a worse prognosis.

Urgent dental care in the Brazilian public health system: learning lessons from the COVID-19 pandemic for future situations / Atendimento odontológico de urgência no sistema público brasileiro de saúde: lições da pandemia da COVID-19 para situações futuras / La atención odontológica de urgencia en el sistema de salud pública brasileño: las enseñanzas de la pandemia de COVID-19 para situaciones futuras

This ecological study described the effect of the COVID-19 pandemic and socioeconomic development on the use and profile of urgent dental care (UDC). UDC rates per 100,000 inhabitants before (from March to June 2019) and during (from March to June 2020) the COVID-19 pandemic in 4,062 Brazilian municipalities were compared. Data were collected from official sources. COVID-19 mortality and hospitalization rates were indicative of levels of lockdown and Human Development Index (HDI) indicated socioeconomic development. Multiple logistic regression and relative excess risk due to interaction (RERI) were used for statistical analyses. The Student t-test was used to compare changes in the profile of UDC causes and procedures in the two periods. Lower UDC rates were found in 69.1% of municipalities and were associated with higher HDI (OR = 1.20; 95%CI: 1.01; 1.42). Mortality had OR = 0.88 (95%CI: 0.73; 1.06) for municipalities with HDI < 0.70 and OR = 1.45 (95%CI: 1.07; 1.97) for municipalities with HDI > 0.70. RERI between HDI and COVID-19 was 0.13 (p < 0.05). Municipalities with greater primary health care coverage had a smaller reduction in emergency rates. Endodontic treatment and dental pain were the most frequent factors both before and during the pandemic. The percentage of UDCs due to pain and soft tissue damage, as well as temporary sealing and surgical procedures, increased. Socioeconomic variables affected UDC rates during the most restrictive period of the COVID-19 pandemic and should be considered in the planning of health actions in future emergencies.

Este estudo ecológico descreveu como a pandemia da COVID-19 e o desenvolvimento socioeconômico afetaram o uso da assistência odontológica de urgência (AOU) e seu perfil. Comparamos taxas de AOU para cada 100 mil habitantes antes (de março a junho de 2019) e durante (de março a junho de 2020) a pandemia da COVID-19 em 4.062 municípios brasileiros. Os dados foram coletados de fontes oficiais. As taxas de mortalidade e internação pela COVID-19 indicaram níveis restritivos de lockdown e Índices de Desenvolvimento Humano (IDH) indicou o nível de desenvolvimento socioeconômico. Foram utilizadas regressões logísticas múltiplas e risco relativo de excesso devido à interação (RERI) para análises estatísticas. O teste t de Student foi usado para comparar alterações no perfil das causas e procedimentos da AOU nos dois períodos. As taxas de AOU foram menores em 69,1% dos municípios e associadas a IDH maior (OR = 1,20; IC95%: 1,01; 1,42). A mortalidade apresentou uma razão de chances de 0,88 (IC95%: 0,73; 1,06) para municípios com IDH < 0,70 e de 1,45 (IC95%: 1,07; 1,97) para municípios com IDH > 0,70. O RERI entre IDH e COVID-19 foi de 0,13 (p < 0,05). Os municípios com maior cobertura de atenção primária à saúde apresentaram menor redução nas suas taxas de emergência. Procedimentos endodônticos e dor dentária foram os fatores mais frequentes antes e durante a pandemia. O percentual de AOUs aumentou devido à dor, danos nos tecidos moles, vedação temporária e procedimentos cirúrgicos. Variáveis socioeconômicas afetaram as taxas de AOU durante o período mais restritivo da pandemia da COVID-19 e devem ser incluídas no planejamento de ações de saúde em emergências futuras.

Este estudio ecológico describió el efecto de la pandemia de COVID-19 y el desarrollo socioeconómico en el uso y el perfil de la atención odontológica de urgencia (AOU). Se compararon las tasas de AOU por cada 100.000 habitantes antes de la pandemia de COVID-19 (de marzo a junio de 2019) y durante la pandemia de COVID-19 (de marzo a junio de 2020) en 4.062 municipios brasileños. Los datos se recogieron de fuentes oficiales. Las tasas de mortalidad y hospitalización de COVID-19 fueron indicativas de niveles restrictivos de desarrollo socioeconómico e Índice de Desarrollo Humano (IDH). Para los análisis estadísticos se utilizó la regresión logística múltiple y el exceso de riesgo relativo (ERR) debido a la interacción. Se utilizó la prueba t de Student para comparar los cambios en el perfil de las causas y los procedimientos de AOU en los dos períodos. Las tasas de AOU fueron menores en el 69,1% de los municipios y se asociaron con un IDH más alto (OR = 1,20; IC95%: 1,01; 1,42). La mortalidad tuvo una OR de 0,88 (IC95%: 0,73; 1,06) para los municipios con IDH < 0,70 y de 1,45 (IC95%: 1,07; 1,97) para los municipios con IDH > 0,70. El ERR entre el IDH y el COVID-19 fue de 0,13 (p < 0,05). Los municipios con mayor cobertura de atención primaria tuvieron una menor reducción de las tasas de urgencia. Los procedimientos de endodoncia y la causa del dolor dental fueron los factores más frecuentes tanto antes como durante la pandemia. Aumentó el porcentaje de AOU por dolor y daños en los tejidos blandos, así como el sellado temporal y los procedimientos quirúrgicos. Las variables socioeconómicas afectaron a las tasas de AOU durante el periodo más restrictivo de la pandemia de COVID-19 y deberían incluirse en la planificación de las acciones sanitarias en futuras emergencias.

Larvicidal potential of cell wall degrading enzymes from Trichoderma asperellum against Aedes aegypti (Diptera: Culicidae).

Aedes aegypti is a mosquito vector of arboviruses such as dengue, chikungunya, zika and yellow fever that cause important public health diseases. The incidence and gravity of these diseases justifies the search for effective measures to reduce the presence of this vector in the environment. Bioinsecticides are an effective alternative method for insect control, with added ecological benefits such as biodegradability. The current study demonstrates that a chitinolytic enzyme complex produced by the fungus Trichoderma asperellum can disrupt cuticle formation in the L3 larvae phase of A. aegypti, suggesting such biolarvicidal action could be used for mosquito control. T. asperellum was exposed to chitin from different sources. This induction of cell wall degrading enzymes, including chitinase, N-acetylglucosaminidase and ß-1,3-glucanase. Groups of 20 L3 larvae of A. aegypti were exposed to varying concentrations of chitinolytic enzymes induced with commercial chitin (CWDE) and larvae cell wall degrading enzymes (L-CWDE). After 72 h of exposure to the CWDE, 100% of larvae were killed. The same percent mortality was observed after 48 h of exposure to L-CWDE at half the CWDE enzyme mixture concentration. Exoskeleton deterioration was further observed by scanning and electron microscopy. Our findings indicate that L-CWDE produced by T. asperellum reflect chitinolytic enzymes with greater specificity for L3 larval biomolecules. This specificity is characterized by the high percentage of mortality compared with CWDE treatments and also by abrupt changes in patterns of the cellular structures visualized by scanning and transmission electron microscopy. These mixtures of chitinolytic enzymes could be candidates, as adjuvant or synergistic molecules, to replace conventional chemical insecticides currently in use.

Low Serum Zinc Levels and Associated Risk Factors in Hospitalized Patients Receiving Oral or Enteral Nutrition: A Case-control Study.

PURPOSE: To assess whether hospitalization and feeding strategy impact the risk of hypozincemia and associated risk factors. METHODS: In this case-control study, serum zinc levels were compared between inpatients fed oral nutrition (ON) (n = 76) or enteral nutrition (EN) (n = 191) with outpatient controls (n = 1095). FINDINGS: Zinc levels were significantly lower in inpatients receiving EN compared with those receiving ON (P = 0.001). Significant (P < 0.001) ß-values of -11.16 and -17.58 for serum zinc concentrations were found for inpatients receiving ON or EN, respectively, compared with the outpatients. Hospitalization and old age were both independent predictors of zinc deficiency. More than 75% of patients >60 years of age fed EN had a zinc concentration <68.75 µg/dL. Low hemoglobin levels increased the risk of low zinc levels for inpatients receiving EN (P = 0.003) and ON (P = 0.026). Age (P < 0.001), noninvasive mechanical ventilatory support (P = 0.016), and critical care (P = 0.018) were risk factors for hypozincemia in patients receiving ON. Low iron levels were associated with hypozincemia (P = 0.001) in patients receiving EN. IMPLICATIONS: Hospitalization and being >60 years of age were risk factors for zinc deficiency. Intensive care and noninvasive mechanical ventilatory support were risk factors for hypozincemia in hospitalized patients who were fed orally. Low hemoglobin levels increased the risk of low zinc concentrations for inpatients receiving EN and ON, and low iron levels were associated with hypozincemia only after EN.

Fontes de informação sobre saúde mental: revisão sistemática da literatura

Resumo: O objetivo deste estudo foi identificar na literatura as fontes de informação sobre saúde mental (FISM) utilizadas pelas pessoas, bem como discutir as vantagens e desvantagens associadas a fontes específicas a fim de compreender as preferências e suas justificativas. Foi realizada uma revisão sistemática de literatura em cinco bases de dados nacionais e internacionais (PubMed, Pepsic, IndexPsi, Scielo e Lilacs) com a combinação entre dois descritores "fontes de informação" e "saúde mental" em Português, Inglês e Espanhol. No total, foram encontrados 46 trabalhos completos, mas apenas 12 foram selecionados para análise. As informações foram organizadas em duas categorias definidas a priori, com base nos objetivos do estudo: FISM utilizadas por grupos específicos e implicações dos resultados dos estudos analisados na prática. Verificou-se que a Internet e os profissionais da saúde foram as FISM de maior preferência das diferentes amostras analisadas. Contudo, é necessário que os profissionais indiquem aos pacientes FISM confiáveis e expliquem as possíveis limitações sobre cada uma. Conclui-se que, embora a Internet tenha aumentado o acesso a informações sobre saúde mental, o papel dos profissionais continua sendo fundamental na produção de conteúdo confiável.

Abstract: The aim of this study was to identify the sources of information on mental health (SIMH) used by people in the literature, as well as to discuss the advantages and disadvantages associated with specific sources in order to understand the preferences and their explanations. A systematic literature review was carried out in five national and international databases (PubMed, Pepsic, IndexPsi, Scielo, and Lilacs) with the combination of two descriptors "information sources" and "mental health" in Portuguese, English, and Spanish. In total, 46 complete papers were found, but only 12 were selected for analysis. The information was organized in two categories defined a priori, based on the objectives of the study: SIMH used by specific groups and implications of the results of the studies analyzed in practice. We verified that the Internet and the health professional(s) were the most favored SIMH of the different samples analyzed. However, it is necessary for practitioners to point out reliable SIMH for patients and to explain possible limitations of each of these. We concluded that, although the Internet has increased access to mental health information, the role of professionals remains crucial in the production of reliable content.

Resumen: El objetivo de este estudio fue identificar en la literatura las fuentes de información sobre salud mental (FISM) utilizadas por las personas, así como discutir las ventajas y desventajas asociadas a fuentes específicas a fin de comprender las preferencias y sus justificaciones. Se realizó una revisión sistemática de literatura en cinco bases de datos nacionales e internacionales (PubMed, Pepsic, IndexPsi, Scielo y Lilacs) con la combinación entre dos descriptores "fuentes de información" y "salud mental" en Portugués, Inglés y Español. En total, se encontraron 46 trabajos completos, pero sólo 12 fueron seleccionados para análisis. Las informaciones fueron organizadas en dos categorías definidas a priori, con base en los objetivos del estudio: FISM utilizados por grupos específicos e implicaciones de los resultados de los estudios analizados en la práctica. Se verificó que la Internet y el profesional de la salud fueron las FISM de mayor preferencia de las diferentes muestras analizadas. Sin embargo, es necesario que los profesionales indiquen a los pacientes FISM confiables y expliquen las posibles limitaciones sobre cada una. Se concluye que, aunque Internet ha aumentado el acceso a información sobre salud mental, la función de los profesionales sigue siendo fundamental en la producción de contenido confiable.

Production of HPMC-films for lactase administration.

The present work aimed to develop and characterize HPMC-films (HFs) based on hydroxypropylmethylcellulose (HPMC) with the addition of lactase for later application in the reduction of lactose in whole milk. The HFs were produced by the casting technique, with the addition of 1, 3, 5 and 7 g of lactase per 100 g of filmogenic solution. All the formulations presented a high capacity of film formation, but the thickness and time of disintegration increased according to the added lactase concentration. Regardless of the formulation developed, HFs in whole milk, a reduction in the lactose content (~ 80%) occurred, classifying the product as zero lactose. Thus, the HPMC-films with the addition of lactase can be considered as a good alternative for solubilization in foods which have a high concentration of lactose.

Factores asociados al consumo de frutas y verduras en Argentina: un estudio multinivel / Factors associated with the consumption of fruits and vegetables in Argentina: A multilevel study

RESUMEN INTRODUCCIÓN Algunos estudios apuntan a una asociación entre la evolución de la prevalencia de enfermedades crónicas no transmisibles con los patrones alimentarios y la situación nutricional de la población. El objetivo de este estudio fue describir el consumo de frutas y verduras en Argentina y su asociación con factores socioeconómicos, demográficos y de actividad física, a nivel individual, provincial y regional. MÉTODOS Se utilizó un modelo multinivel, con información de la Encuesta Nacional de Factores de Riesgo (2013) y del Censo Nacional (2001 y 2010). RESULTADOS El modelo demostró que ser del sexo femenino, tener más años de edad, ingreso a partir de 4.501 pesos y nivel de instrucción secundario completo o más se asocia de manera positiva al consumo de estos alimentos. En contraste, estar separado/a, divorciado/a, viudo/a o soltero/a y practicar actividad física moderada o baja se asocia negativamente a la ingesta de frutas y verduras. El efecto de la variabilidad explicada a nivel provincial y regional resultó ser mínimo. CONCLUSIONES El trabajo se enmarca en una línea de estudios que señalan que los factores relacionados con la alimentación saludable están vinculados a los entornos sociales y físicos, principalmente a características socioeconómicas del grupo de pertenencia y al contexto en que se vive. En este sentido, sería importante contar con información con mayores niveles de desagregación.

ABSTRACT INTRODUCTION Some studies show an association between the evolution of the prevalence of non-communicable chronic diseases with eating patterns and nutritional situation of the population. The purpose of this research was to describe fruit and vegetable consumption in Argentina and its association with socio-economic, demographic and physical activity factors at individual, provincial and regional levels. METHODS A multilevel model approach was used, with information provided in the Argentina National Survey of Risk Factors (2013) and National Census of Population (200! and 2010). RESULTS The model showed a positive association between fruit and vegetable consumption and being female, being older, having a monthly income over the ARS $450! threshold and having finished secondary school. In contrast, being separated, divorced, widowed or single, and practicing moderate or low physical activity were negatively associated with the intake of fruits and vegetables. The effect of the variability at provincial and regional level turned out to be minimal. CONCLUSIONS This work is part of a series of studies that link factors related to healthy eating with social and physical environments, mainly with socioeconomic characteristics of the peer group and its context. In this sense, it would be important to provide more disaggregated information.

Quality of Life and Graduation in Medicine / Qualidade de Vida e Graduação em Medicina

Abstract: Introduction: In recent years, the quality of life of medical students has been questioned, given the context in which they are inserted. Stress as a result of the number of long hours, difficulty in reconciling academic and personal life, exposure to situations of pain and suffering, have been shown to be the main factors for the decrease in quality of life of these students. The aim of this study was to assess the quality of life of medical students and its associated factors. Method: Cross-sectional study with 419 medical students from a public institution in Goiás. The study was carried out with students over 18 years old and enrolled in the second to the eighth semester of medical school, between 2017 and 2018. Sociodemographic and lifestyle questionnaires and the World Health Organization Questionnaire for Quality of Life - Brief Form (WHOQOL-BREF) were used. Results: The factors related to low quality of life were: female gender, the use of stimulants, thinking about giving up medical school and having comorbidities. Those related to a better quality of life were consuming fruits and vegetables, practicing physical activity, more sleeping time and stress management. Conclusion: This study found a set of factors capable of interfering with the quality of life of these students, suggesting the need for interventions aimed at pedagogical and psychological support.

Resumo: Introdução: Nos últimos anos, tem-se questionado sobre a qualidade de vida dos estudantes de Medicina dado o contexto em que estão inseridos. O estresse como resultado do somatório de carga horária extensa, dificuldade de conciliar a vida acadêmica com a pessoal e exposição a situações de dor e sofrimento tem-se mostrado como o principal fator para a queda da qualidade de vida desses acadêmicos. O objetivo deste estudo foi avaliar a qualidade de vida de acadêmicos de Medicina e seus fatores associados. Método: Trata-se de um estudo transversal realizado com 419 acadêmicos do curso de Medicina de uma fundação pública de Goiás. Foram incluídos alunos a partir de 18 anos e matriculados no curso do segundo ao oitavo período, de 2017 a 2018. Utilizaram-se os questionários sociodemográfico e de hábitos de vida e o World Health Organization Questionnaire for Quality of Life - Brief Form (WHOQOL-bref). Resultados: Os fatores relacionados com a baixa qualidade de vida foram: sexo feminino, utilizar estimulantes, pensar em desistir do curso e possuir comorbidade. Os relacionados com melhor qualidade de vida foram consumir frutas e hortaliças, praticar atividade física, mais tempo de sono e gestão do estresse. Conclusão: Este estudo encontrou um conjunto de fatores capazes de interferir na qualidade de vida desses estudantes, sugerindo a necessidade de intervenções direcionadas ao apoio pedagógico e psicológico.

Avaliação dos egressos do Programa de EducaçãoTutorial (PET) docurso de Odontologia da Universidade Federal de Pelotas / Evaluation of graduates of the Tutorial Education Program (PET) of the Dentistry Course at the Federal University of Pelotas

O objetivo desse trabalho foi avaliar o perfil profissional dos egressos da Faculdade de Odontologia da Universidade Federal de Pelotas,participantes do Programa de Educação Tutorial (PET). Foi realizada uma pesquisa descritiva, de natureza qualitativa e quantitativa, utilizando um questionário eletrônico estruturado eautoaplicável como instrumento de coleta e enviado, via correspondência eletrônica, à todos os egressos que participaram efetivamente do grupo PET e aceitaram participar desta pesquisa. Atividade profissional, níveis de formação e objetivos propostos pelo programa foram avaliados. O total de egressos foi de 89, desde a criação do grupo, em 1992, até o ano de 2019. Com as respostas obtidas (n=59), verificou-se que maior parte dos egressos que aderiram a pesquisa concluíram o curso entre os anos de 2004 até 2018 (59.3%), e apenas 24 (40.6%) em período anterior a 2004. Verificou-se que 25% atuam no serviço público, 44% no setor privado e 22% atuam em ambos. Em relaçãoàtitulação, apenas 4(6,8%)não possuem nenhuma pós-graduação, e 53 (89.8%) atuam na regiãoSul do país, 36 (61%) sãodo sexo feminino e 23 (39%) do masculino. Ao final do questionário, foi perguntado arespeito da influência da participação no Programasobre a trajetória profissional, com uma escala de intensidade progressiva de 1 a 5, gerando 19 respostas com nível 4 e 39respostas com nível 5. Os resultados evidenciaram uma influência muito positiva da participação no Programa na vida profissional de seus ex-participantes, tanto no mercado de trabalho, quanto na vivência de pós-graduação (AU).

This study evaluated the professional profile of graduates of the School of Dentistry at the Federal University of Pelotas, participants of the Tutorial Education Program (PET). A descriptive research was conducted, of qualitative and quantitative nature, using a structured and self-administered electronic questionnaire as collection instrument, sent by email to all graduates who effectively participated in the PET group and agreed to participate in this study. The study assessed the professional activity, traininglevels and objectives proposed by the program. The total number of graduates was 89, since the group was created in 1992 until 2019. The answers obtained (n=59) revealed that most graduates who joined the survey completed the course between the years 2004to 2018 (59.3%), and only 24 (40.6%) in the period before 2004. It was found that 25% work in public services, 44% in the private sector and 22% work in both. Concerning the degree, only 4 (6.8%) did not attend postgraduation education, and 53 (89.8%) work in the Southern region of the country, 36 (61%) are females and 23 (39%) are males. At completion of the questionnaire, the participants were asked about the influence of participation in the Program on their professional career, with a scale of progressive intensity from 1 to 5, retrieving 19 responses with score 4 and 39 responses with score 5. The results evidenced a positive influence from participation in the Program in the professional lives of former participants, both in the job market and in the postgraduation experience (AU).

The relation between adolescents' body mass index and bone age / Relación entre índice de masa corporal y edad ósea en adolescentes

Introduction: adolescents' health and nutritional status is demonstrated in the quality of their growth and development. Bone age (BA) is the skeletal development index, which reflects an individual's maturation and is considered as the best expression of human development. Poor nutritional conditions, which extend for long periods, may influence bone development, causing a delay in BA, in relation to chronological age (CA). The aim of this study is evaluating the relation between adolescents' body mass index and bone age. Methods: cross-sectional study carried out with 172 adolescents. The variables evaluated were body mass index (BMI), CA, BA and maturational stage. The BA was determined by taking digital carpal radiographs. Results: the majority of the adolescents presented an adequate nutritional state (73.9%), 42.5% were classified as initial pubertal, followed by 40.6% as in peak height velocity (PHV), with a difference between the sexes (p < 0.001). PHV adolescents presented higher BA averages in both sexes (p = 0.005). There was a correlation between BMI and BA, with a strong correlation for girls in the pre-pubertal stage (r = 0.998; p < 0.05) and moderate correlation for those in PHV (r = 0.421; p < 0.001). By analyzing the relation of the variables to predict adolescents' BA, it was observed that the CA model, maturational stage and BMI explained the variability noted in BA in up to 50.3%. Conclusion: BMI has a relation with BA. It should be noted that BA is influenced by CA, the maturational stage and BMI

Introducción: la salud y el estado nutricional de los adolescentes se manifiestan directamente en la calidad de su crecimiento y desarrollo. La edad ósea (EO) es el índice de desarrollo del esqueleto, que refleja la maduración del individuo y es considerada como la mejor expresión del estado del desarrollo humano. Las condiciones nutricionales deficientes que perduran durante largos periodos pueden influir en el desarrollo óseo, provocando retraso en la EO en relación a la edad cronológica (EC). El objetivo del estudio es evaluar la relación entre el índice de masa corpórea y la edad ósea de adolescentes. Métodos: estudio transversal realizado con 172 adolescentes. Las variables evaluadas fueron índice de masa corpórea (IMC), EC, EO y etapa de maduración sexual. La EO se determinó a partir de la realización de radiografías carpales de archivo digital. Resultados: la mayoría de los adolescentes presentaban un adecuado estado nutricional (73,9%), el 42,5% estaban clasificados como púber inicial, seguidos del 40,6% que fueron considerados en el pico de la velocidad de crecimiento (PVC), con diferencias entre sexos (p < 0,001). Los adolescentes en PVC presentaron mayores medias de EO, en ambos sexos (p = 0,005). Se observó una correlación entre IMC y EO, con fuerte correlación para las niñas en la etapa prepúber (r = 0,998; p < 0,05) y correlación moderada para aquellas en el PVC (r = 0,421; p < 0,001). Al analizar la relación de las variables para predecir la EO de los adolescentes, se observó que el modelo con IC, etapa maduracional e IMC fue capaz de explicar hasta en un 50,3% la variabilidad observada en la EO. Conclusión: el IMC presenta relación con la EO. Se resalta que en la EO influyen la EC, la etapa maduracional y el IMC

The relation between adolescents' body mass index and bone age.

INTRODUCTION: Introduction: adolescents' health and nutritional status is demonstrated in the quality of their growth and development. Bone age (BA) is the skeletal development index, which reflects an individual's maturation and is considered as the best expression of human development. Poor nutritional conditions, which extend for long periods, may influence bone development, causing a delay in BA, in relation to chronological age (CA). The aim of this study is evaluating the relation between adolescents' body mass index and bone age. Methods: cross-sectional study carried out with 172 adolescents. The variables evaluated were body mass index (BMI), CA, BA and maturational stage. The BA was determined by taking digital carpal radiographs. Results: the majority of the adolescents presented an adequate nutritional state (73.9%), 42.5% were classified as initial pubertal, followed by 40.6% as in peak height velocity (PHV), with a difference between the sexes (p < 0.001). PHV adolescents presented higher BA averages in both sexes (p = 0.005). There was a correlation between BMI and BA, with a strong correlation for girls in the pre-pubertal stage (r = 0.998; p < 0.05) and moderate correlation for those in PHV (r = 0.421; p < 0.001). By analyzing the relation of the variables to predict adolescents' BA, it was observed that the CA model, maturational stage and BMI explained the variability noted in BA in up to 50.3%. Conclusion: BMI has a relation with BA. It should be noted that BA is influenced by CA, the maturational stage and BMI.

INTRODUCCIÓN: Introducción: la salud y el estado nutricional de los adolescentes se manifiestan directamente en la calidad de su crecimiento y desarrollo. La edad ósea (EO) es el índice de desarrollo del esqueleto, que refleja la maduración del individuo y es considerada como la mejor expresión del estado del desarrollo humano. Las condiciones nutricionales deficientes que perduran durante largos periodos pueden influir en el desarrollo óseo, provocando retraso en la EO en relación a la edad cronológica (EC). El objetivo del estudio es evaluar la relación entre el índice de masa corpórea y la edad ósea de adolescentes. Métodos: estudio transversal realizado con 172 adolescentes. Las variables evaluadas fueron índice de masa corpórea (IMC), EC, EO y etapa de maduración sexual. La EO se determinó a partir de la realización de radiografías carpales de archivo digital. Resultados: la mayoría de los adolescentes presentaban un adecuado estado nutricional (73,9%), el 42,5% estaban clasificados como púber inicial, seguidos del 40,6% que fueron considerados en el pico de la velocidad de crecimiento (PVC), con diferencias entre sexos (p < 0,001). Los adolescentes en PVC presentaron mayores medias de EO, en ambos sexos (p = 0,005). Se observó una correlación entre IMC y EO, con fuerte correlación para las niñas en la etapa prepúber (r = 0,998; p < 0,05) y correlación moderada para aquellas en el PVC (r = 0,421; p < 0,001). Al analizar la relación de las variables para predecir la EO de los adolescentes, se observó que el modelo con IC, etapa maduracional e IMC fue capaz de explicar hasta en un 50,3% la variabilidad observada en la EO. Conclusión: el IMC presenta relación con la EO. Se resalta que en la EO influyen la EC, la etapa maduracional y el IMC.

Avaliação do conhecimento de alunos de graduação em Odontologia sobre avulsão dentária / Evaluation of dentistry undergraduate students' knowledge about tooth avulsion

Objetivo: avaliar, através de um questionário, o conhecimento dos alunos de graduação do curso de Odontologia na Universidade Veiga de Almeida sobre avulsão dentária. Material e métodos: um questionário estruturado com formação acadêmica, experiências prévias no assunto, e questões de múltipla escolha sobre o conhecimento de avulsão dentária foi aplicado nos alunos do 2° ao 8° período. Resultados: verificou-se que 98,2% sabem o que é avulsão dentária e 54,5% já estudaram ou tiveram algum tipo de instrução sobre o assunto, 92% responderam que o reimplante dental é o melhor procedimento em caso de avulsão. No entanto, apenas 31% sabem como proceder e o correto cuidado com o dente avulsionado (41%). Em relação ao armazenamento do dente, 61% armazenariam em frasco com leite fresco. 81% dos participantes acreditam que o dente avulsionado, após reimplantado, deve ser imobilizado. Conclusão: os alunos de graduação em Odontologia da Universidade Veiga de Almeida apresentam conhecimento sobre a definição de avulsão dentária e que o reimplante é a melhor opção para o tratamento. Porém observa-se que diante dos cuidados com o dente avulsionado e o protocolo de atendimento, o conhecimento dos alunos é escasso

Objective: to evaluate, through a questionnaire, the knowledge of undergraduate students of dentistry at Veiga de Almeida University about dental avulsion. Material and Methods: a structured questionnaire with academic background, previous experience on the subject, and multiple choice questions about knowledge of tooth avulsion was applied to undergraduate students of dentistry from the 2nd to the 8th semester. Results: 98.2% of students knew what is dental avulsion and 54.5% have studied or had some kind of education on the subject, 92% answered that dental replantation is the best procedure in case of avulsion. However, only 31% effectively knew how to proceed and proper care of the avulsed tooth (41%). Regarding tooth storage, 61% would store in a bottle of fresh milk. 81% of participants believe that the avulsed tooth, after reimplantation, should be immobilized. Conclusion: undergraduate dentistry students at Veiga de Almeida University present knowledge about the definition of tooth avulsion and that replantation is the best option for treatment. However, it is observed that due to the care of the avulsed tooth and the care protocol, students' knowledge is scarce

Chemical composition, larvicidal activity and residual effect of Pterodon polygalaeflorus (Benth.) Benth. (Fabaceae) fruit oil extracts against Aedes aegypti (Diptera: Culicidae)

The purpose of this study was to investigate the larvicidal activity of Pterodon polygalaeflorus oil extract against the mosquito vector Aedes aegypti. For this, crushed P. polygalaeflorus fruit underwent solvent extraction to obtain the oil extract. The chemical characterization was performed by gas chromatography coupled to mass spectrometry. For the bioassays third instar larvae of Ae. aegypti were utilized. Tests were carried out to determine the larvicidal activity and the residual effect under laboratory conditions, as well as field screening (small scale). The major components of P. polygalaeflorus oil extract were, respectively, E-caryophyllene, germacrene D and bicyclogermacrene. Lethal concentrations of 50% and 90% were 36.5 and 64.8 ?g/mL respectively. The solution presented a residual effect for seven days and the efficiency of the product was preserved under field conditions. The results encourage continuing studies with the oil extract of P. polygalaeflorus as a research target for bioinsecticides.